在生物科研、農產品及種子檢驗和進出口商品檢驗檢疫等領域,經常需要對將植物組織進行研磨以獲得DNA、RNA或蛋白質等生物大分子,從而進行后續的分析和檢測。隨著實驗室科研進度的日益加快,手動研磨已無法滿足科研工作的需要,越來越多的科研人員都開始使用植物組織研磨器進行研磨,不僅省時省力,也更簡單高效。其中,目前比較普及的研磨機器大都基于研磨鋼珠高速碰撞原理,最常見的主要有2大類,一類是凈信快速組織研磨儀,代表機型如某公司生產的Tissuelyser/JX-FSTPRP系列的自動組織研磨器,另一類則是常規的研磨機器,比較有代表性的是德國某品牌組織研磨機。那么這2類機器有哪些性能特點呢?在使用上有無區別?研磨效果方面有哪些差異?來自實驗的您,又該如何選擇機型呢?本文以上面2種代表機型做參考進行比較,對這些問題做一個簡要總結:
那么如何選擇適合自己的機型呢?
選擇常規研磨機理由:需要一次研磨處理大于200個微量樣品,或者一次處理超大體積樣品時;不需要冷凍研磨。
選擇凈信快速組織研磨儀理由:習慣在實驗室臺面上操作;一次處理量在200個微量樣品以下,同時需要研磨較大體積或質地堅硬的樣品;需要經常進行冷凍研磨;
關于價格方面,就目前了解到的信息,凈信快速植物組織研磨儀與國產常規的研磨機器的價格相差不大(后者均價略貴),而兩種都有不同型號配置的機器,所以定價不一,但市場價位一般在3-6萬左右,相比同類進口機型動輒10萬以上的高價,實則優惠較多。實際上,無論技術與質量,目前國產和進口研磨機器的差距已經非常小,而在現貨以及售后方面,國產研磨機器則具有明顯優勢,畢竟省去了漫長的貨期和高昂的維修費是廣大用戶喜聞樂見的。而出于愛國主義情懷,筆者當下也更傾向于選擇國產機器。當然,最終選擇凈信快速組織研磨儀還是常規組織研磨器必須結合實驗室的實際需求與具體預算綜合考慮了。
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