實驗目的:
多樣品組織研磨機研磨導兔子腿骨,提取DNA。
實驗地點:
首都醫(yī)科大學附屬友誼醫(yī)院
實驗材料和器具
樣品:兔子腿骨
儀器:TISSUELYSER研磨機(上海凈信,TISSUELYSER-24)
(上海凈信,TISSUELYSER-24)
配件:不銹鋼研磨罐2個(上海凈信),
研磨珠(上海凈信)
研磨條件:冷凍干磨
實驗步驟
1. 將凈信研磨罐置于液氮預冷備用
2.將兔子腿骨放在液氮中幾分鐘,然后剪成小塊備用。
3.將小段的腿骨放在研磨罐里,蓋好蓋子。
4.將裝好腿骨的研磨罐從液氮中取出裝入研磨儀,擰緊旋鈕。
4.設定好參數(shù),啟動儀器至研磨程序結束后再次預冷模塊重復程序幾次。
(樣品實驗前后對比)
Previous:關于菠蘿果實采用全自動快速研磨儀研磨提取脂肪酸的一種方法-上海凈信
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