目的:通過研磨脫脂豬皮樣品,對樣品細胞進行提純,研究其藥物作用。破碎過程中脫脂豬皮組織需要在低溫中進行,樣品本身較為堅韌,處理過程比較繁瑣,所以客戶選擇使用上海凈信研磨儀進行前處理破碎。
注意事項:
1、實驗時需要將脫脂豬皮破碎成較小的碎片;
2、實驗過后研磨罐及撞針可用酒精或在超聲波清洗機中進行清洗消毒,烘干備用
3、實驗過程中,樣品的體積不能超過研磨罐的三分之一,留出足夠的空間讓撞針充分運動
實驗地點:愛美客技術(shù)發(fā)展股份有限公司
實驗材料和器具:
樣品:豬皮組織
工具:浸入式液氮冷凍研磨儀(JXFSTPRP-MinniCL)
耗材:鋼罐,液氮
實驗步驟:
1、準備研磨罐一套,處理好的豬皮組織;
2、取處理好的組織樣品放入研磨罐中,將蓋子壓上;
3、首先將研磨罐放入研磨儀中固定,扣好安全鎖,而后將研磨儀整個放入液氮中進行預冷(時間十分鐘)。設置參數(shù)啟動,程序結(jié)束后即可得到破碎完成的豬皮組織樣品;
7、完成后續(xù)對組織樣品細胞的提純步驟。
研磨效果圖:
實驗解決的問題:
1、儀器研磨相對于手工研磨工作時更省力方便,實驗更安全,研磨速度更快;
2、儀器研磨出料顆粒相較于手工研磨更細膩均勻,出料量也有明顯提升;
3、對樣品保護好,提取出的組織成分在數(shù)量和質(zhì)量上比手工研磨效果更好;
4、不會出現(xiàn)交叉感染,實驗更省心
產(chǎn)品介紹:
凈信便攜式液氮冷凍研磨儀功能強大,是世界上技術(shù)領先的液氮冷凍研磨設備,全程-196度低溫下研磨粉碎,是市場上公認的高效的研磨設備:其冷凍研磨方法是一種先進的、高效的、方便的樣品處理方法,可以處理常規(guī)方法難以處理的樣品,如:塑料、橡膠、樹脂、皮革、毛發(fā)、骨骼、木材、紡織原料等等,被稱為研磨的終極手段。液氮冷凍研磨儀應用領域廣泛,如生物化學中的RNA/DNA檢測、聚合物結(jié)構(gòu)分析、痕跡元素的判斷、食品分析、礦物質(zhì)研究、醫(yī)療外科手術(shù)的輔助、藥品性能的檢測、紡織品組成成分的鑒定等等。
優(yōu)勢與特點:
1,低溫狀態(tài)下研磨,保持了生物物質(zhì)活性,確保易揮發(fā)物質(zhì)的保留,防止熱不穩(wěn)定化合物的受熱降解,對熱和機械壓力敏感的代謝物、異構(gòu)體和復雜化合物保持原有的敏感特性物質(zhì)。
2,樣品處理均勻一致、重復性好。
3,研磨參數(shù)設置后的確定性,使樣品處理具有可比性。
4,樣品處理方便、快捷、高效。
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